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応用生態工学 14(2), 115-122, 2011

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原著論文 ORIGINAL PAPER

ECEロゴマーク

セキショウモ(Vallisneria asiatica)の抗酸化機能に及ぼす銅イオン の影響

? 愛民1)*・久場 隆広2)・井芹 寧3)・張 振家4)・劉 玉賢5)・原口 智和6)

1)九州大学東アジア環境研究機構 〒819-0395 福岡市西区元岡744
2)九州大学大学院工学研究院環境都市部門 〒819-0395 福岡市西区元岡744
3)西日本技術開発(株)環境部 〒810-0004 福岡市中央区渡辺通1-1-1
4)上海交通大学環境科学与工程学院 200240 中国上海市海路東川800
5)九州大学大学院工学研究院都市環境システム工学専攻 〒819-0395 福岡市西区元岡744
6)佐賀大学農学部生物環境科学科 〒840-8502 佐賀市本庄町1

Aimin HAO1)*, Takahiro KUBA2), Yasushi ISERI3), Zhenjia ZHANG4), Yuxian LIU5), Tomokazu HARAGUCHI6) : Effects of copper ion on antioxidant function of Vallisneria asiatica. Ecol. Civil Eng. 14(2), 115-122, 2011.

1)Research Institute for East Asia Environments, Kyushu University, 744 Motooka, Nisi-ku, Fukuoka, Fukuoka 819-0395, Japan
2)Department of Urban and Environmental Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nisi-ku, Fukuoka, Fukuoka 819-0395, Japan
3)West Japan Engineering Consultants, Inc., 1-1-1 Watanabe-dori, Chio-ku, Fukuoka 810-0004, Japan
4)School of Environmental Science & Engineering, ShangHaiJiaoTong University, 800 Dongchuan Road Shanhai 200240, China
5)Department of Urban and Environmental Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nisi-ku, Fukuoka, Fukuoka 819-0395, Japan
6)Department of Environmental Sciences, Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga 840-8502, Japan

Abstract: The objective of this research is to demonstrate the antioxidant ecological response of Vallisneria asiatica to the heavy metal Cu2+ pollution which is recently a serious problem in the lakes. Batch experiments with five different concentrations of Cu2+ (0, 5, 10, 20, 40, 80mg・L-1) were carried out. After the 12th day, the roots and leaves of Vallisneria asiatica dipped in the solution with Cu2+ were removed and the physiological and biochemical indexes, chlorophyll, protein and malondialdehyde(MDA) content, superoxide dismutase (SOD), catalase(CAT) and peroxidase(POD) activity were measured. After the experiment; the roots and leaves cultivated in the 0mg・L-1 of Cu2+ group showed chlorophyll and protein content reduced while the MDA content, POD and SOD activity were increased notoriously. Compared with the 0mg・L-1 of Cu2+ group, the 5mg・L-1 of Cu2+ group showed chlorophyll and protein content increase, SOD and POD activity showed also a slight increase, while MDA content decreased. However,for the 10mg・L-1 of Cu2+ group, the decrease of chlorophyll and protein content and the increase of MDA content were observed. Also a sharp increase of SOD and CAT activity compared with the 5mg・L-1group were found; for over 10mg・L-1 of Cu2+ concentration, chlorophyll content and protein content fell off, whereas increase of MDA became considerable, CAT raised up steadily and POD showed a decrease trend. The results showed that, both insufficiency and overabundance of Cu2+ in water environment can affect the anti-oxidation function of Vallisneria asiatica, which can be observed by the value of SOD, CAT and POD activity. Therefore, the measurement of anti-oxidation activity, protein content and MDA as a forecast and judgment indicator of Cu2+ contaminations in the water environment can be considered in the future.

Key words: antioxidant enzyme, copper ion, Vallisneria asiatica

摘要

本研究は,湖沼において重金属Cu2+汚染によるセキショウモ(Vallisneria asiatica) の抗酸化的生理情報を得るため,Cu2+濃度0,5,10,20,40,80mg・L-1の6処理区を設け,処理12日目に根と葉のクロロフィル,タンパク質とマロンジアルデヒド(MDA) の含量及びスーパーオキシドジスムターゼ(SOD),カタラーゼ(CAT) とペルオキシダーゼ(POD)活性を測定した.
実験処理前に比べ,Omg・L-1区では,根と葉ともにクロロフィルとタンパク質含量の減少,MDA含量の増加,SODとPOD活性の上昇が大きかった. 5mg・L-1区では,Omg・L-1区より根と葉ともクロロフィルとタンパク質含量の増加,MDA含量の減少,SODとPOD活性の上昇も少なかった.しかし,10mg・L-1区では,根と葉のクロロフィルとタンパク質含量の減少,MDA含量の増加,根のSODとPOD及び葉のSODとCAT活性の上昇が5mg・L-1区よりも大きかった.さらに20,40,80mg・L-1区では,10mg・L-1区以上に根と葉ともCu2+農度の高くなる区ほど,クロロフィルとタンパク質の減少,MDAの増加が大きくなり,CATが上昇し続け,PODが低下に転じた.
これらの結果により,セキショウモにおいては,水環境のCu2+欠乏及び、Cu2+汚染ともSOD,CATとPOD活性からみた抗酸化機能に影響することが示され,これらの抗酸化酵素活性がクロロフィル,タンパク質とMDAと共に水環境のCu2+汚染の予測と評価の指標になると考えられる.

line 2011年5月16日受付, 2011年10月13日受理
* e-mail: hao.aimin.849@m.kyushu-u.ac.jp line
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